Abstract
DEVELOPMENT AND VALIDATION OF SIMULTANEOUS EQUATION METHOD FOR ESTIMATION OF METRONIDAZOLE BENZOATE AND RELATED IMPURITY IN BULK AND PHARMACEUTICAL FORMULATION
Tabassum H. Aaraf*1 and Hasumati A. Raj2
ABSTRACT
A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Metronidazole Benzoate and related impurity in bulk and pharmaceutical formulation by simultaneous equation method. The Metronidazole Benzoate shows max absorbance at 310.09 nm, 2-Methyl-5Nitroimidazole show max absorbance at 300 and Benzoic Acid shows max. Absorbance at 225.81 nm. The method was found to be linear (r2>0.999) in the range of 2.0-10 ?g/ml. The limit of determination was 0.079 ?g/ml and 0.055 ?g/ml and0.043 ?g/ml for Metronidazole Benzoate, 2-Methyl-5-Nitroimidazole and Benzoic Acid, respectively. The limit of quantification was 0.263 ?g/ml and 0.184 ?g/ml and 0.132 ?g/ml for Metronidazole Benzoate,2-Methyl-5-Nitroimidazole and Benzoic Acid, respectively. The accuracy of this method was evaluated by recovery studies and good recovery result was obtained greater than 99%. The method was successfully applied for simultaneous determination of Metronidazole Benzoate, 2-Methyl-5-Nitroimidazole and Benzoic Acid.
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