Abstract
INVITRO EVALUATION OF METHOTREXATE AND ITS DERIVATIVE (DL-METHOTREXATE (+)- AMETHOPTERIN) FOR THE TREATMENT OF INTRAOCULAR (EYE) MELANOMA
Dr. Syed Ahmed Hussain*, Juveria Gaffar, Farahanaaz Begum, Shaik Nazma Sultana and Sara Umm E. Hani
ABSTRACT
This research paper presents a study on the effects of DL-Methotrexate(+)-Amethopterin on cell viability and functionality. DL-Methotrexate(+)-Amethopterin is a medication used for its anti-inflammatory and anti-proliferative properties, similar to Methotrexate. In this study, we employed multiple assays to investigate the impact of DL-Methotrexate(+)-Amethopterin on cell behavior. The assays included MTT Assay, Tubulogenesis Assay, Indirect Immunofluorescence Assay, and Western Blot Analysis. The results of these assays provide insights into the influence of DL-Methotrexate(+)-Amethopterin on cell viability and its potential implications in the context of therapeutic applications.
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