World Journal of
Pharmaceutical and Life Sciences

HPLC CHROMATOGRAPHY

*Salim Bala Muhammad and Atul Tiwari

ABSTRACT

Chromatography is an analytical technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. The various constituents of the mixture travels at different speed, causing them to separate. High Performance Liquid Chromatography is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Each component in the sample interacts slightly differently with the adsorbent material, causing different flow rates for the different component and leading to the separation of the component as they flow out the column.

[Full Text Article]   [Download Certificate]