World Journal of
Pharmaceutical and Life Sciences

ANALYTICAL METHOD DEVELOPMENT VALIDATION OF MEROPENEM AND VABORBACTAM OF PURE AND DOSAGE FORMS USING RP-HPLC METHOD

K. Mounika*, L. Ramachandra Reddy and D. Dhachinamoorthi

ABSTRACT

A simple, Accurate, precise method was developed for the simultaneous estimation of the Meropenem and Vaborbactam in Tablet dosage form. Chromatogram was run through Agilent C18 (150 x 4.6 mm, 5?) Mobile phase containing Buffer 0.01N KH2PO4: Methanol taken in the ratio 50:50 was pumped through column at a flow rate of 0.8 ml/min. PH adjusted to 5.0 with dil. Orthophosphoric acid solution. Temperature was maintained at 30°C. Optimized wavelength selected was 260 nm. Retention time of Meropenem and Vaborbactam were found to be 2.1119 min and 2.654 min. %RSD of the Meropenem and Vaborbactam were and found to be 0.7 and 0.7 respectively. %Recovery was obtained as 99.52% and 100.04% for Meropenem and Vaborbactam respectively. LOD, LOQ values obtained from regression equations of Meropenem and Vaborbactam were 0.07, 0.21 ?g/ml and 0.07, 0.21 ?g/ml respectively. Regression equation of Meropenem is y = 8848.x + 698.1, and y = 8748.x + 998.1of Vaborbactam. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.

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