World Journal of
Pharmaceutical and Life Sciences

ANTI MICROBIAL ACTIVITY OF Ehretia Laevis Roxb. (KHANDU CHAKKA) PLANT

Dr. Thakre Rushikesh*, Khobragade Pramod and Harne Ketaki

ABSTRACT

Local peoples from Vidarbha(MS) India are using paste of leaves of this plant for wound healing since many years and known as Khandu Chakka. Study is planned to assess the antimicrobial activity of leaves of this plant against gram positive and gram negative organism responsible for wound infection. Which will be helpful to provide the cheap convenient and alternative medicine for wound healing. Plant is confirmed by taxonomist. Ethanolic and water extracts are done by soxhlet extractor. Crude extract is collected in all aseptic precaution. Nutrient agar medium for bacterial strains will be used for the antimicrobial activity. Amoxicillin drug will be used as a standard for antimicrobial activity. Antibacterial Activity is done by Agar well diffusion method. Size of zones (in mm) will be observed for different concentration. ( in ?g/ml) i. e. 1000 ?g/ml, 500 ?g/ml, 250 ?g/ml, 100 ?g/ml, 50 ?g/ml, 10 ?g/ml. Amoxicillin, the standard compound have shown the maximum zone of inhibition against both the selected species of microorganism i.e. S. aureus and E. coli. at all the concentrations. Ethanolic extract was found to be ineffective against E. coli at all concentrations. The zone of inhibition produced by the Ethanolic extract on S. aureus was significant at lower concentration but did increase significantly with increase in the concentration of extract. The moderate antimicrobial activity was produced by crude and water extract at all concentrations against E. coli when compared with the Amoxicillin. Conclusion- Antimicrobial activity of Crude and water extract of Khandu Chakka of was found to be better in S. aureus as compared to E. coli. Antimicrobial activity of Crude and Water extract of Khandu Chakka was found to better on S. aureus as compared to ethanolic extract.No antimicrobial activity was found in Ethanolic extract on E.coli. Anti microbial activity of water extract of Khandu Chakka was found to be best amongst three extracts.

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