World Journal of
Pharmaceutical and Life Sciences

A CARBON TETRACHLORIDE INDUCED HEPATOTOXICITY IN RABBITS: EVALUATION OF CURATIVE ROLE OF TURKISH PANAX GINSENG

Sadiq S. Mareai*, Kamal A. Al-Samawi, Fawaz A. Al-Monifi

ABSTRACT

Aims: The present study aimed to evaluate the effect of Turkish Panax Ginseng (TPG) on hepato-marker enzymes and biochemical parameters in animal models (rabbits) with carbon tetrachloride (CCl4) - induced hepatotoxicity at a single dose of 1.25 ml/kg body weight as a mixture with olive oil. Main methods: Intoxication of rabbits by CCl4 significantly increased (p < 0.01) serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), serum alkaline phosphatase (SALP) and bilirubin level, which indicate acute hepatocellular damage and biliary obstruction. Intoxicated animals were treated orally with TPG at (100, 200 and 300 mg/kg bodyweight) and Liv-52 (25 mg/kg) for 28 consecutive days. The hepatocellular damage was assessed by measuring the changes of SGOT, SGPT, SALP, total bilirubin, total protein, creatinine, blood urea nitrogen (BUN) and blood glucose level using rabbits weighing 1.2-1.5 kg. Key findings: In vivo results indicated oral administration with TPG could overcome CCL4-induced immunosuppression and significantly (p < 0.05) exhibited protective effect. The results of TPG treated rabbits showed a significant dose-dependent reduction (P< 0.05) in the hepatic enzymes levels, bilirubin, BUN, blood glucose level and improvement of serum total protein when compared with that of Liv-52. Highest activity was observed for TPG at 300 mg/kg body weight dose level and the reductions of SGOT, SGPT, SALP, total bilirubin, BUN and creatinine in serum were 55.50, 52.43, 59.02, 57.32, 31.21 and 20.8%, respectively. In addition, TPG increased the serum total protein level by 3 folds, when compared with CCl4 treated rabbits. Significance: TPG possess antihepatotoxic action and may be acting as a natural hepatoprotective agent against CCl4 induced hepatocellular injury. This might be due to its active antioxidant contents and higher amount of active ginsenosides. TPG could effectively attenuate the alteration within the studied parameters in dose-dependent manner and prevent oxidative damage in immunological system.

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