World Journal of
Pharmaceutical and Life Sciences

ACTIVITY OF LACTOBACILLUS ACIDOPHILUS, L. PLANETARIUM, STREPTOMYCES AND SACCHAROMYCES CEREVISIAE WITH EXTRACTS OF DATE PALM AND DRIED SHELL OF POMEGRANATE TO REDUCE AFLATOXIN M1 IN IRAQ

Mohsen Hashim Risan, Sulaf .H .Taemor*, Athraa H. Muhsin** Saja M. Hafied,
Sarah H. Ghayyib, Zahraa H. Neama

ABSTRACT

This study aimed to evaluate the efficiency of the bioproduct of the bacteria Lactobacillus acidophilus, L. Planetarium, Streptomyces and Saccharomyces cerevisiae with extracts of date palm and dried shell of pomegranate to reduce aflatoxin M1 produced by Aspergillus flavus. The antifungal activities of microbes and extracts were evaluated using the disk diffusion and agar well diffusion methods, the inhibitory zones were recorded in millimetres. The results showed that the population of fungi was higher in milk spoilt samples, three isolates diagnosed as Aspergillus flavus A1, A2 and A3. The results of this study showed that all treatment used against Aspergillus flavus A2 were showed antifungal, inhibition zone reached of treatment 5 -19.3 mm. The results of this study were encouraging, despite the need for clinical studies to determine of the real effectiveness and potential toxic effects in vivo. These results were revealed the importance of some microbial and plant extracts in control of aflatoxin M1. Using thin layer chromatography (TLC) method, show results generally reveal that all starters were characterized by their ability to gradually degrade AFM1.

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