World Journal of
Pharmaceutical and Life Sciences

ALCOHOLIC PAIRED YOYO® BITTERS INDUCES LEYDIG CELL ATYPIA IN RATTUS NOVERGICUS

Eric E. U., Oboma Yibala I.*, Indighe Ditimilaemi A., Onuoha Chinedu E. and Beredugo Sylvanus

ABSTRACT

YOYO ® bitters cleanser is Nigeria certified herbal products by National Agency for Food, Drugs and Control (NAFDAC) as the first non-alcoholic, coloring or artificial preservatives bitters produced in Nigeria and therefore alcoholic yoyo bitters is a modified form by end users called combined. This study was carried out to investigate the effect of alcoholic yoyo bitters on the histomorphology of the testis induced with binge alcohol drinking. Twenty four (24) adult male Wistar rats were randomly selected into three (3) groups n=8. Group A=control received 1.5ml of vita-milk and water per kg-1b.wt, Group B received 1.5mLof 30% alcoholic vita- milk per kg- 1b.wt, and Group C received 1.5mL of 30% alcoholic vita- milk and 0.1mL of YOYO ® bitters cleanser per kg- 1b.wt by oral route. The substance was administered three times for four days daily and the animals were sacrificed on the 5th day by cervical dislocation and the testes excised following abdominal incision for histological observation using H/E stain. Blood samples was collected into EDTA and plain tubes for hematology and clinical chemistry respectively. Results showed degeneration of the germinal epithelium of the seminiferous tubule and widening of the interstitial space involving loss of Leydig cell compared with control. There was equally decreased in serum testosterone (ng/ml) level, Ca2+, Mean cell volume(MCV), and total Calcium was reduced while total protein and chloride ions was reduced in the alcoholic yoyo bitter group compared with the normal and was statistically significant at P<0.05. Alcoholic paired yoyo bitters has been confirmed as toxic to sperm cells. This interesting and comprehensive study showed that binge alcoholism alone and in combination with YOYO ® bitters alters testicular function and morphology and therefore alcoholics taking YOYO ® bitters for treatment of other ailments not investigated in the present work should avoid its combination with yoyo bitters to reduce male infertility.

[Full Text Article]   [Download Certificate]