World Journal of
Pharmaceutical and Life Sciences

COMPARISON BETWEEN DIFFERENT DIAGNOSTIC METHODS TO DETERMINE THE HOSPITAL PREVALENCE OF THEILERIA ANNULATA INFECTION IN CATTLE-CALVES IN BIKANER

*Pavan Goyal, Anju Chahar, S. S. Soni and B. N. Shringi

ABSTRACT

A total of 100 cattle-calves irrespective of their age, sex and breed brought to Teaching Veterinary Clinical Complex of College of Veterinary and Animal science, Bikaner for treatment were screened for Theileria annulata infection. Blood samples were collected from jugular vein aseptically in ethylene diamine tetra acetic acid (EDTA @ 1 mg/ml) vacutainer for genomic DNA isolation and isolated DNA samples were subjected to nested polymerase chain reaction (nPCR) using primer sets. Blood smears were prepared from ear vein for detection of Theileria piroplasms and lymph node aspirate smears were prepared from the enlarged superficial lymph nodes for detection of schizont stage in mononuclear cells under oil immersion lens. Out of 100 samples, only 7 were positive for intra-erythrocytic piroplams, 3 for schizont stage in mononuclear cells upon microscopic examination while 41 samples were found positive for Theileria annulata infection by nPCR which produced 372 bp amplicon on 1% agarose gel. Thus, the hospital prevalence of Theileria annulata infection by Giemsa stained blood smear examination, lymph node aspirate smear examination and nPCR was 7%, 3% and 41%, respectively. Hence, nPCR showed significantly higher efficacy in detection of Theileria annulata infection as compared to microscopic examination.

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