World Journal of
Pharmaceutical and Life Sciences

ACTIVITY OF GULURONATE LYASE IN THE ENHANCED PRODUCTION OF BACITRACIN A IN THE LIQUID CULTURES OF BACILLUS LICHENIFORMIS NCIMB 8874

Constancy Prisca Aleru*, Ebirien-Agana Samuel Bartimaeus, Maryam Safari and Tajalli Keshavarz

ABSTRACT

This research reports for the first time the presence and the activity of guluronatelyase in the elicited (using oligoguluronate) liquid cultures of Bacillus licheniformis in the enhanced production of bacitracin A. Oligoguluronate (OG) prepared by partial acid hydrolysis of sodium alginate was added aseptically in concentrations ranging from 50 to 200 mg/L to cultures of Bacillus licheniformis (control cultures and cultures at 0hour (50 mg/L) and 24hour (200 mg/L)). Enhanced production of bacitracin A was observed at 32hour fermentation period with the highest production of bacitracin A obtained in the 0hour cultures (when 50 mg/L of OG was added at 0 hour) followed by the 24hour cultures to which 200 mg/L of OG was added. Results also showed, for the first time, that the enzyme guluronate lyase is produced in the cultures of Bacillus licheniformis when OG is added to the cultures and that the increase in the activity of this enzyme was concomitant with the bacterial growth, protein concentration of the cultures and the enhanced production of bacitracin A. The discovery of the presence of this enzyme (guluronatelyase) in the enhanced bacitracin A production from cultures of B. licheniformis could lead to potential applications of the methodology for large-scale production of bacitracin A in the future.

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