World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
World Journal of Pharmaceutical and Life Sciences (WJPLS) has indexed with various reputed international bodies like : Google Scholar, Index Copernicus, Scientific Indexing Services (SIS), Indian citation Index (ICI), Directory of Research Journals Indexing, Scintific journal impact factorScientific Journal Impact Factor (SJIF), Jour Informatics (Under Process), UDLedge Science Citation Index, Global Impact Factor (In Process), International Impact Factor Services, International Society for Research Activity (ISRA) Journal Impact Factor (JIF), Science Library Index, Dubai, United Arab Emirates, International Innovative Journal Impact Factor (IIJIF), Scientific Journal Impact Factor (SJIF), Eurasian Scientific Journal Index (ESJI), Cosmos Impact Factor, IFSIJ Measure of Journal Quality, International Scientific Indexing, UAE (ISI), Web of Science Group (Under Process), Research Bible, Fuchu, Tokyo. JAPAN, Scholar Article Journal Index (SAJI), Scope Database, Academia, 

Abstract

METHOD DEVELOPMENT AND VALIDATION OF EZETIMIBE BY RP-HPLC IN BULK AND THE PHARMACEUTICAL DOSAGE FORM

Elkaturi Sanjeeth*, Gajavelli Santhosh, Kampati Ravi and Duddagi Suchitra

ABSTRACT

A simple, rapid, precise, accurate and highly sensitive High Performance Liquid Chromatography method has been developed and validated for the determination of Ezetimibe in bulk and pharmaceutical dosage form. Chromatographic separation was achieved on INERTSIL, C18 (150×4.6 ID) 5?m column using mobile phase consisting of a mixture of Acetonitrile and H20 in the ratio of 80:20 and the flow rate was adjusted to 1ml/min with UV detection at 232nm. The retention time of Ezetimibe was found to be 3.560 min. Linearity was observed in the range of 20-60 ?g/ml for Ezetimibe. In the linearity study, the regression equation and coefficient of correlation for Ezetimibe was found to be (y=6.416x+16.91, r²=0.998).The proposed method was validated according to ICH Q2B guidelines. The accuracy of the method was assessed by recovery studies at three different levels. Recovery experiments indicated the absence of interference from commonly encountered pharmaceutical additives. The method was found to be precise as indicated by the repeatability analysis, showing %RSD less than 2. All statistical data proves validity of the method and can be used for routine analysis of pharmaceutical dosage form.

[Full Text Article]   [Download Certificate]

WJPLS CITATION

  All Since 2019
 Citation  422  322
 h-index  9  7
 i10-index  4  2

INDEXING

NEWS & UPDATION

    A PHP Error was encountered

    Severity: Notice

    Message: Undefined variable: news

    Filename: views/right_panel.php

    Line Number: 79

    A PHP Error was encountered

    Severity: Warning

    Message: Invalid argument supplied for foreach()

    Filename: views/right_panel.php

    Line Number: 79

BEST PAPER AWARDS

World Journal of Pharmaceutical and life sciences Will give best paper award in every issue in the from of money along with certificate to promote research .

Best Article of current issue

Download Article : Click here