World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

GC-MS ANALYSIS AND ANTIMICROBIAL ACTIVITY OF SAUDI TRIGONELLA FOENUM L. (FABACEAE) FIXED OIL

Abdel Karim M.*, Ayeda Y. and Khalid M. S.

ABSTRACT

In this study, Trigonella foenum seed oil was analyzed by GC-MS and antimicrobial potency of the oil was evaluated. 26 components were detected by GC-MS analysis being dominated by: z,z-9,12-octadecadienoic acid(35.85%), 9,12,15-octadecatrienoic acid methyl ester(19.66%),hexadecanoic acid methyl ester(13.29%), z-9-octadecenoic acid methyl ester (10.13%). The antimicrobial activity of the oil was evaluated via the diffusion bioassay against five standard human pathogens(Gram positive: Staphylococcus aureus and Bacillus subtilis; Gram negative : Escherichia coli and Pseudomonasa aeruginosa and the fungal species Candida albicans) . The oil showed different antimicrobial responses against test organisms. The oil showed excellent activity against the bacterial strain Staphylococcus aureus in the concentration range: 100-25mg/ml. It also exhibited excellent activity against the yeast Candida albicans at 100mg/ml. The oil showed activity against all test organism at 100 mg/ml.

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