World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF BENZOXONIUM CHLORIDE AND LIDOCAINE HYDROCHLORIDE

Dr. Santosh V. Gandhi* and Ashwini D. Ighe

ABSTRACT

The aim of the present study was to develop and validate reverse phase high performance liquid chromatography (RP-HPLC) method for simultaneous estimation of Benzoxonium chloride and Lidocaine Hydrochloride in bulk and combined dosage form. An isocratic, RP-HPLC method was developed on Nucleosil C18 (250 x 4.6 mm, 5 ?m) column using 10 mM potassium dihydrogen phosphate buffer and acetonitritle (20:80 v/v) as mobile phase at flow rate of 1 ml/min at detection wavelength of 215 nm. The chromatographic conditions yield good separation between drugs with retention time (RT) of 5.28 ± 0.13 min and 9.76 ± 0.36 min for Lidocaine hydrochloride and Benzoxonium chloride, respectively. The method was validated with respect to linearity, precision, accuracy and robustness. The data of linear regression analysis indicated a good linear relationship over the range of 20-120 ?g/ml for both drugs with a correlation coefficient (r2) of 0.998 for Benzoxonium Chloride and 0.996 for Lidocaine hydrochloride. The developed method was found to be simple, sensitive, selective, accurate and repeatable for simultaneous analysis of Benzoxonium chloride and Lidocaine hydrochloride and can be adopted for routine analysis of these drugs in bulk and pharmaceutical dosage form.

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