World Journal of
Pharmaceutical and Life Sciences

ISOLATION OF SAFE TISSUE GLUE AND MOLECULAR FINGERPRINTING OF HAMMOUR (GREASY GROUPER) FISH

Adel E. EL-Tarras, Ahmed shahaby, Alaa A. Mohamed, Dr. Mohamed M. Hassan* and Ahmed Gaber

ABSTRACT

Saudi Arabia fisheries resources are quite rich with diverse fish species. This diversity of fish species is important to changes in aquatic environment (e.g. pollution, global warming and overfishing...etc) which in turn might led to numerous consequences for fish populations. Collagen is a major structural protein of connective tissues. It can be used as a prosthetic biomaterial applicable to artificial skin, tendon ligaments and development collagen implants. In the present study, an attempt was made to isolate and characterize collagen from hammour fish. Hammour, or grouper, is the most popular eating fish in Saudi Arabia due to its deliciousness and rich source of protein. During its processing, the scales, Fins, Skins etc are expelled out as waste acid solubilized collagen. Initial extraction by this method acid yielded 20-30% of collagen on dry weight basis. SEM micrograph showed the fibrous nature of this collagen. Moreover, the molecular characterization of Saudi Arabia grouper fish was done using RAPD, rep-PCR and ISSR markers. Specific results for fingerprinting were obtained by the several primers of each RAPD, rep-PCR and ISSR analysis produced different fragment patterns with varied number of bands. The primers yielded a total of 176 distinct bands 72.2% were considered as polymorphic bands and 27.8% were considered as monomorphic bands. The Dendrogram based on RAPD, rep-PCR and ISSR results grouped the twelve grouper fish samples into two different clusters with about 82% genetic similarity.

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