World Journal of
Pharmaceutical and Life Sciences

THE EFFECTS OF AMIKACIN ON RED BLOOD CELL MEMBRANE STABILITY

Aiman Ehtisham, Ruqaiya Hasan*, Mahanoor Nisar, Maira Hasan

ABSTRACT

Background: Physicians widely use Amikacin as an aminoglycoside antibiotic to treat severe gram-negative
infections. However, researchers must further evaluate its interaction with non-targeted cells, such as erythrocytes.
Objective: To evaluate the impact of Amikacin on erythrocytes membrane integrity and hemolysis under
controlled in vitro conditions. Materials and Methods: Washed human red blood cells were treated with
increasing doses of Amikacin (25–200 µL/mL). Samples were maintained at 37 °C for 30 minutes, and red blood
cells lysis was measured by spectrophotometer at 540 nm. Structural changes were evaluated microscopically.
Results: Erythrocytes destruction enhanced in a dose-related manner, with negligible impact at lower
concentrations and considerable cell destruction at higher concentrations. Microscopy confirmed progressive
structural alterations in red blood cells. Conclusion: The disruption of erythrocytes at high doses of Amikacin,
highlighting the importance to evaluate the drug - cell reaction.

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