World Journal of
Pharmaceutical and Life Sciences

FORMULATION DESIGN AND IN VITRO EVALUATION OF HERBAL VESICLES CONTAINING HERBAL MEDICINAL PLANT EXTRACT BY QBD

Ankit Kumar*, Dharmendra Singh Rajpur, Brajmohan Kausnal

ABSTRACT

The present study aimed to formulate and evaluate herbosomes of Amaranthus viridis extract using a Design of Experiments (DOE) approach to optimize the formulation process. The extraction of Amaranthus viridis was followed by the preparation of herbosomes, which were evaluated for their percentage yield, and the results revealed a yield of Amaranthus viridis in Pet ether and Ethanol were 0.79% and 0.85%. Phytochemical analysis confirmed the existence of bioactive substances such alkaloids, flavonoids, saponins, and phenols, indicating the therapeutic potential of the extract. The formulation of the extract-loaded herbal vesicle (herbosomes) involved optimizing dependent variables (e.g., lipid-to-extract ratio, surfactant concentration) and independent variables using DOE software. The optimization process significantly improved the formulation's characteristics. The resulting herbosomes were characterized for physical properties, in addition to particle size distribution analysis revealed an average size of 112 nm. Zeta potential analysis demonstrated a stable formulation with a potential value of 28.5 mV, indicating good dispersion stability. SEM analysis provided insights into the morphology of the herbosomes, showing a spherical vesicle structure. Stability studies conducted at various temperatures and conditions confirmed that the herbosomes remained stable at intervals of 30, 60, and 90 days (3 month). Furthermore the ability of the antimicrobial herbosome formulation was assessed using the well diffusion assay, which showed significant inhibitory effects against E. coli and S. aureus, suggesting strong antimicrobial potential. Overall, the study demonstrated the successful formulation of Amaranthus viridis-based herbosomes with promising stability and antimicrobial properties.

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