World Journal of
Pharmaceutical and Life Sciences

PHARMACOLOGICAL EVALUATION, ANTI-OXIDANT AND WOUND HEALING ACTIVITY OF POLYHERBAL FORMULATION CONTAINING LEAF EXTRACTS OF EUPHORBIA HIRTA AND BRYOPHYLLUM PINNATUM

Abdul Kalam*, Rahul Dubey, Dr. Bala Subramanian

ABSTRACT

The present study aimed to develop and evaluate topical herbal gels containing Euphorbia hirta and Bryophyllum pinnatum extracts for their phytochemical profile, antibacterial activity, and wound healing potential. Qualitative phytochemical screening confirmed the presence of active constituents such as alkaloids, flavonoids, glycosides, saponins, phenols, and proteins in both extracts. Quantitative estimation of total phenolic content (TPC) and total flavonoid content (TFC) was carried out using gallic acid and rutin as standards, respectively. Acute toxicity studies revealed no signs of toxicity up to 2000 mg/kg body weight, indicating the safety of the extracts. The prepared gels exhibited acceptable physicochemical characteristics, with pH values ranging from 5.9 to 6.6, suitable viscosity, and good spreadability without causing skin irritation. Antibacterial evaluation by well diffusion assay demonstrated that the polyherbal gel (Formulation III) exhibited the highest zone of inhibition (25 mm) against Escherichia coli. Wound healing studies using an excision wound model in rats showed significant improvement in wound contraction with the polyherbal gel compared to individual plant formulations and the control. The enhanced activity is attributed to the synergistic effect of phytoconstituents from both plants. These findings suggest that the Euphorbia hirta and Bryophyllum pinnatum combination gel may serve as a promising topical agent for wound management.

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