World Journal of
Pharmaceutical and Life Sciences

COMPARATIVE STUDY OF MICROPROPAGATION OF EUGENIA SP.NOV.1 AND SYZYGIUM PARKERI (MYRTACEAE) UNDER THE INFLUENCE OF BENZYLAMINOPURINE (BAP), NAPHTHALENE ACETIC ACID (NAA) AND POTASSIUM PHOSPHATE DEHYDROGENATE (NaH2PO4)

Ravoniarison Nivohanintsoa Elinorovololona* and Rakotomalala Ariliva Mireille

ABSTRACT

Some researches about the micropropagation of Eugenia sp.nov.1 and Syzygium parkeri (Myrtaceae) threatened of extinction at the mining site of Ambatovy in Madagascar were carried out in order to manage their multiplication and preservation. Surface sterilization tests of the explants, using CaOCl2 at 5% or 7% concentration and soaking time of 10 or 15min, were performed. In vitro regeneration of these species were established on MS/2 medium supplemented with 150ml/l of green coconut water, 2g/l of activated charcoal, 0 or 90mg/l of potassium phosphate dehydrogenate (NaH2PO4) and 0.5 to 3mg/l of BAP and/or 0 to 0.5mg/l NAA. The aseptic culture establishment using 7% of calcium hypochlorite (CaOCl2) during 10min allowed to obtain the best rate of survival explants (20% of contamination rate and 0% of death rate). 2mg/l of BAP and 90mg/l of NaH2PO4 produced 3 shoots/explants for Eugenia sp.nov.1 and Syzygium parkeri. An average length of 2cm of the shoots was obtained in the medium growth regulators free. As for the in vitro rooting, different reactions were observed for the two species. 1.5mg/l of BAP permitted to get an average of 0.5 root/explant for Eugenia sp.nov.1. For Syzygium parkeri, 1.6 roots/explant were obtained with 3mg/l/0.2mg/l of BAP/NAA in combination.

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