World Journal of
Pharmaceutical and Life Sciences

FORMULATION AND EVALUATION OF MICROSPHERE CONTAINING COMBINATION OF ANTI-PIGMENTATION DRUGS CREAM FORMULATION AND ITS ANTI-MICROBIAL ACTIVITY

Amandeep Singh*, Pinky Singh, Hemant Agarwal

ABSTRACT

The present study focuses on the formulation and evaluation of a microsphere-based cream containing mometasone furoate and hydroquinone for topical application. Mometasone furoate appeared as an odorless off-white solid powder, while hydroquinone was identified as a white, odorless crystalline powder. Solubility studies indicated that both drugs dissolved readily in ethanol, dimethyl sulfoxide, and methanol. The pH of mometasone furoate and hydroquinone was found to be 4.4 and 3.8, respectively, with melting points of 173°C and 221°C. UV spectrophotometric analysis revealed λmax values of 250 nm for mometasone furoate and 293 nm for hydroquinone, within specification limits. Microspheres were prepared and characterized by particle size, zeta potential, drug entrapment efficiency, and morphology. The average particle size ranged between 150.4 and 217.8 nm, with zeta potential values of 0.3–9.1 mV, indicating good stability. Drug entrapment efficiency ranged from 77.03% to 95.86%, with the F1 formulation showing the highest entrapment. Scanning electron microscopy confirmed spherical microspheres with smooth surface morphology. The optimized microspheres were incorporated into a cream base, which demonstrated favorable physicochemical properties including viscosity (4823±0.31 cps), spreadability (12.70 g·cm/s), and skin-compatible pH (6.6). Antimicrobial testing showed significant inhibition of bacterial growth, confirming the formulation’s effectiveness against microbial skin conditions. Overall, the developed microsphere-based cream improved the solubility, stability, and skin penetration of mometasone furoate and hydroquinone while demonstrating notable antimicrobial activity. These findings suggest that the formulation holds strong potential as an effective and patient-compliant topical treatment for inflammatory and microbial-associated skin disorders.

[Full Text Article]   [Download Certificate]