World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

A REVIEW ARTICLE ON ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF METFORMIN AND SITAGLIPTIN IN BULK AND FIXED DOSE COMBINATION

Pratiksha Anil Ithape*, Vaishnavi Sunil Jadhav, Mr. Shirsat Ajaykumar

ABSTRACT

This article provides an overview of the simultaneous estimation of the combination drugs metformin and sitagliptin using the RP-HPLC method. Reverse phase chromatography is the most widely utilized separation technique in HPLC due to its simplicity, versatility, and capability to manage compounds with varying polarity and molecular weight. A solid understanding of the various types of mobile phases and their combinations is essential for developing a highly precise and accurate method. The retention time and linearity of metformin and sitagliptin were evaluated under different chromatographic conditions, which include factors such as column type, mobile phase, elution mode, flow rate, and the wavelength detected with a UV detector. In this article, we will review various methods that have been developed to estimate the combination of these drugs using RP-HPLC.

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