World Journal of
Pharmaceutical and Life Sciences

LC–MS–DRIVEN QUANTITATIVE AND MECHANISTIC EVALUATION OF VINORELBINE IN EYE CANCER CELL LINE MODELS

Somabatthini Shruthi*, Dr. Syed Ahmed Hussain, Ghousia Begum, Nada Ahmed Al Amoodi, Fariya Sultana, Bilquis Begum, Ayesha Ayub Khan, Muskan Khatoon

ABSTRACT

This study evaluates the comparative in vitro cytotoxic and viability profiles of Vinorelbine and Vinblastine in eye cancer cell line models, including retinoblastoma (Y79, WERI-Rb1) and uveal melanoma (OCM-1, 92.1). A five-assay panel was employed, comprising two viability assays (Resazurin/Alamar Blue, ATP Luminescence) and three cytotoxicity assays (Annexin V/PI, Caspase-3/7 activity, LDH release). Vinorelbine demonstrated significant reduction in viability (58% and 52%) compared to Vinblastine (100% in both assays), indicating potent antiproliferative activity. Cytotoxicity assays revealed strong apoptotic induction by Vinorelbine (45% apoptotic cells, 2.8-fold caspase activation, 47% LDH release), while Vinblastine showed negligible cytotoxic effects (7%, 1.0-fold, 8%, respectively). These results highlight Vinorelbine’s enhanced pro-apoptotic potency and capability to disrupt membrane integrity, suggesting robust activation of programmed cell death pathways. Overall, Vinorelbine exhibits greater therapeutic potential than Vinblastine in ocular tumor models, warranting deeper mechanistic and translational evaluation.

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