World Journal of
Pharmaceutical and Life Sciences

LC–MS–BASED ANALYTICAL PROFILING AND CYTOTOXIC EVALUATION OF VINCRISTINE IN EYE CANCER CELL LINE MODELS

Dr. Syed Ahmed Hussain*, Somabatthini Shruthi, Ghousia Begum, Nada Ahmed Al Amoodi, Fariya Sultana, Bilquis Begum, Ayesha Ayub Khan, Muskan Khatoon

ABSTRACT

This study evaluates the comparative in vitro cytotoxic and viability effects of Vinblastine and Vincristine across eye cancer cell line models, including retinoblastoma (Y79, WERI-Rb1) and uveal melanoma (OCM-1, 92.1). A five-assay panel was designed, comprising two viability assays (Resazurin/Alamar Blue and ATP Luminescence) and three cytotoxicity assays (Annexin V/PI, Caspase-3/7 activity, and LDH release). Vinblastine maintained full viability (100%) with minimal apoptotic induction (7% apoptotic cells, 1.0-fold caspase activation, and 8% LDH release), reflecting negligible cytotoxic stress. In contrast, Vincristine significantly reduced viability (40–36%) while inducing strong apoptosis (60%), a 3.9-fold increase in caspase activity, and 62% LDH release, indicating robust activation of programmed cell death pathways. These findings highlight Vincristine’s potent pro-apoptotic and cytotoxic activity in ocular tumor models, whereas Vinblastine demonstrated primarily cytostatic behavior. The results support further exploration of Vincristine as a strong apoptotic agent in retinoblastoma and uveal melanoma treatment paradigms.

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