World Journal of
Pharmaceutical and Life Sciences

EFFECT OF NITROGEN APPLICATION ON PHANEROCHAETE CHRYSOSPORIUM ENZYME ACTIVITY AND WHEAT STRAW BIODEGRADATION IN DIFFERENT WHEAT GENOTYPES

Arvind Kumar*, Girish Chandra Pandey, Pooja Rani

ABSTRACT

Wheat straw, a major agricultural byproduct, presents a challenge for disposal and an opportunity for valorization through biodegradation. This study investigates the impact of varying nitrogen application rates on the enzymatic activity of the white rot fungus Phanerochaete chrysosporium, specifically Laccase and Manganese Peroxidase (MnP), and their role in the biodegradation of straw from five different wheat genotypes (C306, DPW621-50, HD2967, DBW303, and DM7). The experiment was conducted over a period of 45 days, with measurements taken at 15, 30, and 45-day intervals. Three nitrogen application rates were tested: N0 (0 kg/acre), N1 (120 kg/acre), and N2 (240 kg/acre). The results show that enzyme activities varied significantly across genotypes and were strongly influenced by nitrogen levels and time. Notably, the N2 treatment generally led to the highest enzyme activities for both Laccase and MnP, particularly at the 45-day mark. This suggests that higher nitrogen availability enhances the biodegradative potential of Phanerochaete chrysosporium on wheat straw. These findings have significant implications for sustainable agricultural practices and the management of crop residues.

[Full Text Article]   [Download Certificate]