World Journal of
Pharmaceutical and Life Sciences

ANTIBIOFILM ACTIVITY OF CITRIC ACID AGAINST DUAL BACTERIAL BIOFILMS

*Dr. Anuya Aparna Rege 

ABSTRACT

Both E. coli and Salmonella species could adhere to various food-contact surfaces and produce biofilms leading to recurrent contamination and foodborne outbreaks. Nearly all biofilms found in nature are made up of mixed species. Therefore, there is urgent need for effective antibiofilm agent targeting multi-bacterial biofilms. Hence, in the present in vitro study, Citric Acid was evaluated for its Antibiofilm potential against Dual Bacterial Biofilms of E. coli, S. enterica and S. typhi with different bacteria using crystal violet staining method. Citric Acid exerted the highest antibiofilm effect against Dual bacterial biofilms of E. coli + S. enterica followed by E. coli + E. faecalis > E. coli + MRSA > E. coli + S. typhi > E. coli + P. aeruginosa > S. enterica + MRSA > S. typhi + MRSA > S. typhi + E. faecalis > S. enterica + E. faecalis > S. enterica + S. typhi > S. enterica + P. aeruginosa > S. typhi + P. aeruginosa. Combination prepared from Citric Acid (CA) and Ciprofloxacin (CP) was also evaluated against total 12 Dual bacterial biofilms that showed somewhat similar antibiofilm activity to that of Citric Acid alone. In our previous studies, Citric Acid has displayed antibiofilm potential against Mono and Mixed Bacterial biofilms as well as against Dual and Polymicrobial biofilms of C. albicans with different Bacteria [Fungus + Bacteria], whereas, in present in vitro study, Citric Acid has revealed antibiofilm potential against Dual bacterial biofilms [Bacteria + Bacteria]. Therefore, Citric Acid could be used as an effective antibiofilm agent to treat microbial biofilms related menaces be it foodborne diseases or nosocomial infections.

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