World Journal of
Pharmaceutical and Life Sciences

DEVELOPING AN EFFICIENT PROTOCOL FOR RAPID IN VITRO MULTIPLICATION OF VANILLA PLANIFOLIA ANDREWS (ORCHIDACEAE) OF MADAGASCAR

Harinarivo Hoby Lalatiana*, Rakotoarisoa Noronirina Victorine, Rabemanantsoa Christian, Tombozara Nantenaina, Razafindrakoto Rinah Zoarilala, David Ramanitrahasimbola, El-Jaziri Mondher and Andrianjara Charles

ABSTRACT

Under natural conditions, the lack of endosperm limits the orchid’s germination including Vanilla sp. Only 1 to 2% of orchids germinate under difficulty after an extensive period of dormancy. This work aims to find out the efficient in vitro protocol to speed up Vanilla planifolia Andrews (Orchidaceae) strains propagation. Two in vitro methods were applied during this work such as the asymbiotic germination using different pods stages development of vanilla seeds and the nodal segments propagation using different MS media in combination with various phytohormones. High rate protocorms of 87.28% were induced with mature pods of 9 months using MS basal medium supplemented with 2 mg/ml each of NAA and BAP and that of MS/2 medium supplemented with 1% of Myo-inositol and 0.1% Thiamine-HCl was identified the best to develop 100% of plantlets from protocorms. MS/2 basal medium supplemented with 1 mg/ml of BAP and 1 mg/ml of Kin was the most efficient for nodal culture by developing in 5 days of incubation only the first node and giving liana with 6 nodes at 45 days after cultivation, reaching 77%.These methods could be used and tested for the same species of V.planifolia and to others strains for a rapid multiplication and development under in vitro conditions.

[Full Text Article]   [Download Certificate]