World Journal of
Pharmaceutical and Life Sciences

EFFECT OF IMMUNOSTIMULANT AND AEROMONIASIS ON THE BIOCHEMICAL ASPECTS OF MUSCLE IN 9 MOHTHS OLD LABEO ROHITA

*Ankamma N. and Viveka Vardhani V.

ABSTRACT

Feeding with supplementary diet (Aqua Fix) and/or infection with Aeromonas hydrophila explored marked biochemical changes and immune response in the muscle of Labeo rohita. Nine months old fish (3 groups) were used in the present investigation. Groups A and B were treated with Aqua Fix for 4 days, on day 5 fish of group B and C were infected with Aeromonas hydrophila @ 10-6CFU/fish(fish of group C were fed with normal diet for 4 days).Controls (group D) were untreated and uninfected. Necropsies were made on day 1, 2,3,4,7 and 15 after infection in fish of groups B and C; fish of group A and D were also necropsied on the same designated days. Estimated muscle protein, carbohydrate, DNA and RNA were analysed by statistical method and found that Aqua Fix is able to induce protective immune response with regard to enhance the level of muscle protein, carbohydrate, DNA and RNA. Abnormal levels of protein, carbohydrate, DNA and RNA in fish infected with A.hydrophila (group C) indicates the lowered immunity due to the stress caused by the pathogen.

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