World Journal of
Pharmaceutical and Life Sciences

EVALUATION OF THE LIPASE-PRODUCING POTENTIAL OF BACILLUS SUBTILIS AND PSEUDOMONAS AERUGINOSA FROM VITELLARIA PARADOXA LEAVES

Ademola O. R.*, Oladipo I. C., Ogunsona S. B., Ogunleke O. B. and Akinwumi T. M.

ABSTRACT

Lipases are industrially important enzymes that are widely used for food product processing, drug processing, biodiesel production, and the bioremediation of contaminants. The main objective of this study was to isolate and identify bacteria that produce lipase from Vitellaria paradoxa leaves and to evaluate their enzymatic capabilities. Initial screening was conducted using Rhodamine-olive oil agar plates, where the appearance of orange fluorescent halos under UV light indicated lipase activity. Strains that tested positive underwent additional molecular characterization via 16S rRNA gene sequencing, which confirmed their identities as Bacillus subtilis and Pseudomonas aeruginosa. Preliminary activity tests revealed that both strains could produce lipase in laboratory conditions; however, each showed distinct enzyme activity patterns when exposed to different pH levels and temperature variations. These initial results suggest that both strains could be refined for targeted industrial applications.

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