World Journal of
Pharmaceutical and Life Sciences

ADVANCES IN IN-VITRO SCREENING FOR SQUAMOUS CELL CARCINOMA: INSIGHTS INTO DRUG DEVELOPMENT AND EFFICACY

Ayesha Munawar, Sumaiyya Mehreen* and Syed Majid

ABSTRACT

Aim: This study aims to evaluate the cytotoxic effects of the epidermal growth factor receptor (EGFR) inhibitor, N-benzyl-3-chloro 4-fluoroaniline (N-(3-ethynylphenyl)-6,7-bis(2-methoxyethoxy)quinazolin-4-amine), in comparison with the chemotherapeutic agent Cisplatin in kidney cancer cell lines. Objective: The primary objective is to assess the efficacy of N-benzyl-3-chloro 4-fluoroaniline against various concentrations (1, 5, and 10 µM) and to determine its impact on cell viability using multiple assays, including MTT, CellTiter-Glo, Alamar Blue, SRB, and LDH cytotoxicity assays. Research: The study utilized five different assays to measure cell viability and cytotoxicity, analyzing the responses of kidney cancer cell lines to N-benzyl-3-chloro 4-fluoroaniline and Cisplatin. Results indicated a concentration-dependent decrease in cell viability for both drugs, with N-benzyl-3-chloro 4-fluoroaniline showing significant cytotoxic effects, particularly at higher concentrations. The MTT assay results revealed cell viability percentages of 87.3%, 65.1%, and 39.6% for N-benzyl-3-chloro 4-fluoroaniline at 1, 5, and 10 µM, respectively, compared to the control. Conclusion: N-benzyl-3-chloro 4-fluoroaniline demonstrates promising cytotoxic effects against kidney cancer cell lines, warranting further investigation into its clinical potential as a therapeutic option, particularly in combination therapies with Cisplatin.

[Full Text Article]   [Download Certificate]