World Journal of
Pharmaceutical and Life Sciences

IN-VITRO EVALUATION OF CELL VIABILITY STUDIES OF KIDNEY CANCER USING SIMILAR MOLECULE – PAZOPANIB

Dr. Syed Ahmed Hussain*, Maimuna Fatima, Umaima Batool Osmani, Arshiya Tarannum, Faheem Unnisa, Raheem Unnisa Shaik and Nazneen

ABSTRACT

Aim: The study aims to investigate the cytotoxic effects of Pazopanib on kidney cancer cells using a series of in
vitro assays. Objective: The primary objective is to assess the dose-dependent inhibition of kidney cancer cell
viability by Pazopanib and compare its activity with the control compound, Everolimus. Research: Kidney cancer
cells were treated with increasing concentrations (1 μM, 5 μM, and 10 μM) of Pazopanib. The MTT, CellTiter-
Glo, Alamar Blue, SRB, and LDH Cytotoxicity assays were employed to evaluate cell viability. Results from the
MTT assay revealed a reduction in cell viability from 79% at 1 μM to 39% at 10 μM. Similar trends were observed
in the CellTiter-Glo, Alamar Blue, and SRB assays, which further confirmed Pazopanib’s dose-dependent efficacy.
However, the LDH Cytotoxicity Assay exhibited an increase in cell viability at 10 μM concentration, suggesting
potential alternative mechanisms of cell death. Conclusion: Pazopanib exhibited strong dose-dependent inhibition
of kidney cancer cell viability across multiple assays, indicating its potential as a therapeutic candidate. Further
research is necessary to fully understand its mechanism of action, particularly in relation to the results obtained
from the LDH assay.

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