World Journal of
Pharmaceutical and Life Sciences

ADVANCEMENTS IN THIN-LAYER CHROMATOGRAPHY: A COMPREHENSIVE EXPLORATION

Dr. Osman Ahmed* Khadeeja and Dr. Anas Rasheed

ABSTRACT

Thin-Layer Chromatography (TLC) has evolved into a versatile and robust analytical technique, demonstrating its
enduring relevance in the dynamic landscape of analytical chemistry. This comprehensive exploration delves into
the principles, recent innovations, and diverse applications that have shaped the trajectory of TLC from its
inception to the present day. The fundamental principles of TLC, grounded in the separation, identification, and
quantification of compounds within a mixture, provide the foundation for recent developments aimed at enhancing
efficiency and reliability. Innovations in stationary phases, including modified silica, reversed-phase materials, and
chiral phases, address limitations in separation selectivity, broadening the applicability of TLC to diverse
compounds. Instrumentation and automation have witnessed transformative advances, with modern TLC systems
equipped with robotic applicators, controlled development chambers, and precise detection mechanisms, offering
enhanced efficiency and high-throughput capabilities. High-performance TLC (HPTLC) techniques have further
elevated resolution and sensitivity, making TLC a competitive choice in various analytical scenarios.

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