World Journal of
Pharmaceutical and Life Sciences

STATISTICAL OPTIMIZATION OF LIPASE PRODUCTION USING USED COOKING OIL (UCO) AS A CHEAP CARBON SOURCE FROM PSEUDOMONAS AERUGINOSA SJ2

Joshi Swapnil Satish*

ABSTRACT

Pseudomonas aeruginosa SJ2 isolated and characterized in previous studies was used for lipase production using used cooking oil (UCO) as a cheap carbon source. Before optimization Pseudomonas aeruginosa SJ2 was able to produce 300 ± 4 U/mL of lipase in production medium consisting of (g/L; yeast extract (2.0), UCO (40.0 mL/L), NaCl (2.0), MgSO4 (0.4), (NH4)2SO4 (0.5), K2HPO4 (0.3), and KH2PO4 (0.3), pH 8.0 ± 0.2), glucose (0.5%), triton-X 100 (0.05%), temperature 30 °C, incubation time 48 h). Statistical optimization using central composite design (CCD) resulted in production of 329 ± 3 U/mL of lipase under optimized conditions viz. (g/L; yeast extract (2.33), UCO (49.0 mL/L), NaCl (2.0), MgSO4 (0.4), (NH4)2SO4 (0.5), K2HPO4 (0.3), and KH2PO4 (0.3), pH 8.0 ± 0.2), glucose (0.5%), triton-X 100 (0.05%), temperature 32 °C, incubation time 48 h). Scale-up studies were performed in a 3.0 L bench top fermenter (Systronics India Ltd., Ahmedabad) with 1.5 L working volume where lipase production dropped marginally to 321 ± 4 U/mL. Overall optimization resulted in 1.1-fold increase in lipase production by Pseudomonas aeruginosa SJ2 using cost effective medium.

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