World Journal of
Pharmaceutical and Life Sciences

APPLICATION OF LIPASE PRODUCED BY PSEUDOMONAS AERUGINOSA SJ2 IN THE SYNTHESIS OF METHYL SALICYLATE

Joshi Swapnil Satish*

ABSTRACT

Purified lipase having specific activity of 2,826 U/mg produced by Pseudomonas aeruginosa SJ2 was used for the synthesis of methyl salicylate an ester. Initially a reference profile of standard methyl salicylate was prepared in DMSO for gas chromatography. Under unoptimized conditions lipase was able to convert 52.69 % of reactants into methyl salicylate. During optimization studies effect of amount of lipase, effect of relative amount of reactants, and incubation time was studied. Maximum methyl salicylate yield of 64.21% was observed when amount of enzyme was 20-30 ?L. Maximum yield of 63.84% of methyl salicylate was achieved at a 1:1 molar ratio (50 mM:50 mM) of reactants and maximum yield of 67.05% was achieved at the end of 12h incubation period. When all the above parameters were optimized maximum conversion of 67.05% was achieved.

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