World Journal of
Pharmaceutical and Life Sciences

COMBATTING ANTIBIOTIC RESISTANCE IN CHRONIC PERIODONTITIS BY DISSEMINATION OF PLASMID IN ENTEROCOCCUS FAECALIS

Usha Subbiah*, Angeline Julius, Athira Ajith and Sonaa Elango

ABSTRACT

This study aimed to investigate the basis of plasmid-mediated resistance to antimicrobials in Enterococcus faecalis isolated from unstimulated saliva and subgingival plaque. In 50 chronic periodontitis samples, E. faecalis (n=34) was isolated from unstimulated saliva and subgingival plaque. The resistant isolates were screened from disc diffusion assay and MIC determination by using the agar dilution method. The bacterial transformation was performed to ensure the susceptibility of the plasmid in the clinical isolates. A conjugation experiment was carried out using the solid agar mating method. In addition, plasmid curing assay was performed to determine plasmid-mediated antibiotic resistance. Salivary and subgingival plaque isolates had maximum resistance to ampicillin (92%), metronidazole (90%) and linezolid (95%). The plasmid-encoded antibiotic resistance reversal was proved from the plating, where acridine orange had a remarkable plasmid curing efficiency of 82% and 87% on ampicillin, and on linezolid resistance 89% and 81% of saliva and plaque E. faecalis respectively. The present study’s findings might allow the eradication of this plasmid-mediated resistance in pathogens during antibiotic adjuvant in periodontal therapy.

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