World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

ASSESSMENT OF ANTIFUNGAL ACTIVITY OF COMBRETUM ROXBURGHII SOLVENT LEAF EXTRACTS AGAINST ASPERGILLUS FLAVUS.

Pragnyasini Rout, Subhashree Mohapatra and Sunita Bhatnagar*

ABSTRACT

Combretum roxburghii belongs to a medicinally important family Combretaceae which is used in traditional medicine against inflammation, infection, diabetes, malaria, bleeding, diarrhoea, cancer and treatment of syphilis abdominal pain. In the present study solvent leaf extracts were explored for antifungal activity against Aspergillus flavus. Four leaf extracts (hexane, chloroform, ethyl acetate and methanol) were prepared. Four extracts were tested at two doses i.e., 1000?g and 2000?g/ml. Hexane and chloroform extract showed mild activity in radical growth method. Methanol extract showed best activity in agar well diffusion method. Chloroform extract showed 45% and 89% reduction in biomass of the fungus in agar diffusion method and liquid broth medium respectively. Ethyl acetate extract reduced the aflatoxin content by 66% when compared with control samples. Thus all the four extracts had antifungal activity in one way or other.

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