World Journal of
Pharmaceutical and Life Sciences

EFFECTS OF X AND GAMMA RAYS ON THE RADIOSENSITIVITY TEST OF TWO MALAGASY UPLAND RAIN FED RICE VARIETIES

Razafinirina Lydia*, Rabefiraisana Jhonny Harimialimalala, Rakotonjanahary Xavier Roland Théophile, Rasoamampionona Berthe, Ratsimiala Ramonta Isabelle, Andrianjaka Alice and Rakotoarisoa Noronirina Victorine

ABSTRACT

Finding the appropriate irradiation dose rate is a critical step before initiating a mutation plant breeding program. The objective of this research is to identify the most effective irradiation type for generating genetic diversity in two Malagasy upland rice varieties, namely 3729 and 3737, which are susceptible to the parasitic plant Striga asiatica. The seeds of these rice varieties were subjected to different irradiation doses, ranging from 0, 75, 150, 300, 450, and 600 Gy, using Gamma and X-rays. The germination and survival rates of the rice seeds were evaluated based on the LD50 value, while the height and root length were assessed using GR30. Based on the LD50 values for germination and survival rates, as well as the GR30 values for shoot and root length reduction, irradiation doses ranging from 75 to 100 Gy were found to be favorable for inducing mutations in the genomes of both rice varieties using X-rays. For gamma rays, the suitable irradiation doses were determined to be between 75 and 200 Gy. It should be noted that gamma irradiation exhibited greater effectiveness in inducing genetic variation in all tested rice varieties compared to X-rays.

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