World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
World Journal of Pharmaceutical and Life Sciences (WJPLS) has indexed with various reputed international bodies like : Google Scholar, Index Copernicus, Scientific Indexing Services (SIS), Indian citation Index (ICI), Directory of Research Journals Indexing, Scintific journal impact factorScientific Journal Impact Factor (SJIF), Jour Informatics (Under Process), UDLedge Science Citation Index, Global Impact Factor (In Process), International Impact Factor Services, International Society for Research Activity (ISRA) Journal Impact Factor (JIF), Science Library Index, Dubai, United Arab Emirates, International Innovative Journal Impact Factor (IIJIF), Scientific Journal Impact Factor (SJIF), Eurasian Scientific Journal Index (ESJI), Cosmos Impact Factor, IFSIJ Measure of Journal Quality, International Scientific Indexing, UAE (ISI), Web of Science Group (Under Process), Research Bible, Fuchu, Tokyo. JAPAN, Scholar Article Journal Index (SAJI), Scope Database, Academia, 

Abstract

EVALUATION OF IN VITRO CYTOTOXIC ACTIVITY OF ETHANOLIC EXTRACT OF ROOT OF IPOMOEA BATATAS (L) (EERIB) AGAINST THE HUMAN COLORECTAL ADENOCARCINOMA HT-29 CELL LINE

Dr. Mukuntha Kumar. N*, Asish Bhaumik, Shubhankar Saha

ABSTRACT

HT-29 is a human colorectal adenocarcinoma cell line with epithelial morphology. These cells are sensitive to the chemotherapeutic drugs 5-fluorouracil and oxaliplatin, which are standard treatment options for colorectal cancer. In addition to being a xenograft tumor model for colorectal cancer, the HT-29 cell line is also used as an in-vitro model to study absorption, transport, and secretion by intestinal cells. Under standard culture conditions, these cells grow as a nonpolarized, undifferentiated multilayer. Altering culture conditions or treating the cells with various inducers, however, results in a differentiated and polarized morphology, characterized by the redistribution of membrane antigens and development of an apical brush-border membrane. The main aim and objective of the present research work was the evaluation of in vitro Cytotoxic activity of ethanolic extract of root of Ipomoea batatas (L) (EERIB) against the human colorectal adenocarcinoma HT-29 cell line. The in vitro cytotoxic activity was carried out by SRB assay. The results obtained from the in-vitro studies performed by SRB assay by using human colorectal adenocarcinoma HT-29 cell line displayed that the EERIB possessed a very good cytotoxic activity. From the present studied it had been concluded that EERIB was exhibiting the potential cytotoxic action on HT-29 cell line which was proved by using standard drug 5-FU and it was found that EERIB showed the highest 90.95% growth inhibition at 10 ?g (IC50 = 2.7 ?g/ml. The IC50 value of standard drug 5-FU was found to be 1.3 ?g/ml with 95.97% growth inhibition at concentration 75 ?g/ml.

[Full Text Article]   [Download Certificate]

WJPLS CITATION

  All Since 2019
 Citation  422  322
 h-index  9  7
 i10-index  4  2

INDEXING

NEWS & UPDATION

    A PHP Error was encountered

    Severity: Notice

    Message: Undefined variable: news

    Filename: views/right_panel.php

    Line Number: 79

    A PHP Error was encountered

    Severity: Warning

    Message: Invalid argument supplied for foreach()

    Filename: views/right_panel.php

    Line Number: 79

BEST PAPER AWARDS

World Journal of Pharmaceutical and life sciences Will give best paper award in every issue in the from of money along with certificate to promote research .

Best Article of current issue

Download Article : Click here