World Journal of
Pharmaceutical and Life Sciences

PRE-CLINICAL EVALUATION OF ANTIDIABETIC ACTIVITY OF PHYLLANTHUS RETICULATUS FRUIT EXTRACT IN STREPTOZOTOCIN-INDUCED DIABETIC RATS

Abhijit Sahana, Pushpendra Ahirwar, Dr. Kunal Gupta*

ABSTRACT

Diabetes mellitus is a metabolic illness with numerous etiologists that is defined by chronic hyperglycemia and abnormalities in carbohydrate, lipid, and protein metabolism caused by insulin production, insulin action, or both. Hyperglycemia is a symptom of diabetes mellitus, which is a collection of disorders. Diabetes mellitus is classified as an epidemic by the World Health Organization (WHO), despite the fact that it is a non-infectious disease. This disease is predicted to affect over 203% of the world's population, with an annual growth rate of 4-5%. The powdered material of the fruit of Phyllanthus reticulates was extracted separately using 95% methanol by Soxhlet apparatus Phyto-constituentsuents like alkaloids, tannins, cardio glycosides, and traces of flavonoids, etc. The dose selection study was carried out in accordance with the OECD 423 guidelines. Female Wistar rats were used in this experiment. There were two groups, each with six fasting animals. A single dosage of the test drug was given to one group of 300 mg/kg and another group of 2000 mg/kg body weight. Any indicators of toxicity should be detected in the animals prior to dosing and for a total of 14 days. Following that, the test dose was selected as 300 mg/kg and 600 mg/kg. Group-I: Normal Control, Group-II: Disease Control (Only Streptozotocin), Group-III: Low Dose of Phyllanthus reticulates Fruit Extract (300mg/Kg Body Weight) + Streptozotocin, Group-IV: High Dose of Phyllanthus reticulatus Fruit Extract (600mg/Kg Body Weight) + Streptozotocin, Group-V: Standard Drug [Metformin] (0.5gm/Kg Body Weight) + Streptozotocin study biochemical parameters such as lipid profile (Total cholesterol, Triglycerides, HDL, LDL).

[Full Text Article]   [Download Certificate]