World Journal of
Pharmaceutical and Life Sciences

THIN-LAYER CHROMATOGRAPHY: A RESEARCH REPORT

Syeda Rakhshinda Zareen*, Dr. Osman Ahmed, Reshma, Mohammed Sayeed Uddin and Dr. Anas Rasheed

ABSTRACT

In this paper, we'll try to break down the fundamentals of Thin-Layer Chromatography (TLC) and its relevance in a variety of analytical procedures. Because of its cheap cost, ease of use, and widespread applicability in the pharmaceutical industry, TLC has recently gained popularity as an analytical method. It is possible to separate all 32 amino acids with TLC, but only with extreme precision. Further, it may be used in a variety of contexts, particularly for the purpose of determining the presence of contaminants in a chemical. Prior to high-performance liquid chromatography (HPLC), it may be employed as a preliminary analytical procedure. TLC is easy to understand conceptually, and it requires little preparation of materials in most cases. TLC is useful for tracking reaction rates and determining which chemicals are present in a sample. Identical substances in a mixture may be separated using TLC as well. TLC is the gold standard in many fields, including industrial chemistry, environmental toxicity, food chemistry, water, inorganic and pesticide analysis, dye purity, cosmetics, plant materials, and herbal analysis.

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