World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Manal A. Ibrahim*Byadir Alhaj Mohammed and Hatil Hashim El-Kamali


Background: The objectives of this work to determine the chemical composition of the essential oil of Nigella sativa from fixed oil of seeds and to evaluate the antioxidant activity of essential oil in vitro. Method: The essential oil was extracted from fixed oil by distillation, then the essential oil was chemically analyzed and identified by Gas Chromatography-Mass Spectroscopy (GC/MS). The essential oil was tested for a possible antioxidant activity by using DPPH method. Results: The principal components of the essential oil was as follows: thymoquinone (57.99%), p-cymene (28.90%), alpha-phellandrene (6.331%), alpha- terpineol (2.706%) and limonene (1.353%). Conclusion: Thymoquinone the major constituents of the Sudanese commercial oil sample, (57.99%) has not recorded in any previous literature in dominant quantities among the major compounds from the oils of N. sativa. The observed compositional difference between N. sativa found in Sudanese commercial sample and other area from the globe could be due to environmental and genetic factors or chemo-types, which can influence essential oil composition.

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