World Journal of
Pharmaceutical and Life Sciences

CHEMOMETRIC ASSISTED RP-HPLC METHOD FOR ACECLOFENAC AND PANTOPRAZOLE ESTIMATION

Shine Sudev*

ABSTRACT

Aceclofenac is (2-2[-[2[2,6 –dichlorophenyl)amino]phenyl] acetyl] oxyacetic acid with a Molar mass: 353.0216g/mol. Aceclofenac is a pain reliever. It treats rheumatoid arthritis, ankylosing spondylitis, and osteoarthritis by reducing pain and inflammation. It is a kind of nonsteroidal anti-inflammatory medication (NSAID). It works by inhibiting the release of particular chemical messengers responsible for pain and inflammation .The medication works by blocking the function of cyclooxygenase, which is involved in the creation of PG, which is responsible for pain, swelling, inflammation, and fever.Pantoprazole is Sodium;5-(difluoromethoxy)-2-[(3,4-dimethoxypyridin-2yl)methylsulfinyl] benzimidazol-1-ide. Pantoprazole is a proton pump inhibitor that reduces acid production in the stomach. It is used to treat erosive esophagitis (esophageal damage caused by gastroesophageal reflux disease, or GERD) in adults and children over the age of 5. The LC18 analytical Supelcosil (Supelco) column was used to achieve the chromatographic conditions (5 micron -LC18, 250x4.6mm) The mobile phase was composed of phosphate buffer (PH 4.3), acetonitrile, and methanol in the following proportions: 50:40:10. The sample was injected at a volume of 20 microliters, and the mobile phase was degassed using an infrared digital ultra sonicator before being pumped into the HPLC system. The flow rate was set at 0.8 ml per minute, and the wave length 284nm was chosen for detection. The column temperature was kept at 30oC.

[Full Text Article]   [Download Certificate]