World Journal of
Pharmaceutical and Life Sciences

PHYTOCHEMICAL ANALYSIS, ANTI OXIDENT, IN VITRO ANTI-CANCER ACTIVITY OF ERYTHRINA VARIEGATA L. LEAVES

T. Marimuthu, R. Manikandan and G. Anburaj*

ABSTRACT

Objective: Erythrina variegata L. species have been reported to be used in the treatment of cancer in traditional/folk medicine, which may explore their anticancer potential. Methods: A preliminary qualitative phytochemical screening presence of flavonoids, phenolic compounds, tannins, terpenoids, carotenoids, chlorophyll and alkaloids. Chemical principles of 1,1-diphenyl-2-picrylhydrazyl (DPPH•) radical scavenging, Fe3+–Fe2+ transformation assay, ferric reducing antioxidant power (FRAP) assay, superoxide radical ion (O2•?), scavenging, superoxidizing assay, hydroxyl Radicals, nitric oxide (NO) scavenging, metal chelating and reducing power function. These various antioxidant activities were compared with standard antioxidants such as L-ascorbic acid. Results: Erythrina variegata leaf extract exhibited the greatest antioxidant activity via DPPH, superoxide anion scavenging, metal chelator (iron chelator and iron reducing power) and nitric oxide scavenging activity. The antioxidant activity of Erythrina variegata leaves the extract due to the presence of flavonoids and polyphenols. Anticancer activity of Erythrina variegata leaves was confirmed against human colon cancer cell line (HT-29). Anticancer activity was directly stimulated by concentrations and maximum growth inhibition was 81.38% at 400 ?g/ml. Conclusion: Overall, it can be concluded from the present study that Erythrina variegata leaves are rich in phytochemicals and natural antioxidants, which have anticancer activity.

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