World Journal of
Pharmaceutical and Life Sciences

PRODUCTION OF CITRIC ACID FROM THE PINEAPPLE WASTE BY USING ASPERGILLUS NIGER

PRODUCTION OF CITRIC ACID FROM THE PINEAPPLE WASTE BY USING ASPERGILLUS NIGER

ABSTRACT

Citric acid is one of the most important organic acids and is generally used in different industries. Pineapple’s peel which had a harder texture and difficult to be eaten, would always ended up as waste. It is anticipated that discarded fruit as well as the waste material can be utilized for further industrial processes like fermentation, bioactive component extraction, etc. The study was carried out to explore the potential of Aspergillus niger for citric acid production from the pineapple waste. In the study the effects of various parameters like temperature, pH, carbon sources and nitrogen sources have also been checked for citric acid production. In the study A. niger was isolated from the rotten onion on potato dextrose agar and identified by morphological and cultural characteristics. The A. niger isolated was further utilize for the production of citric acid using pineapple waste. Pineapple peels were used as a substrate for the production of citric acid by submerged fermentation method. The production was found to be 2.77 g/lit with 41.56 % of citric acid for peels used as a substrate. The maximum production of citric acid was found to be at 25o C temperature, pH 7 with addition of 1 % sucrose and ammonium chloride at 168 hrs of incubation. The present aspect of utilization of pineapple peels for the production of the citric acid can also be helpful in minimizing pineapple waste disposal.

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