World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

EVALUATION OF NYCTANTHES ARBORTRISTIS FOR ANTIFUNGAL ACTIVITY USING ASPERGILLUS FLAVUS AND ASPERGILLUS NIGER AS TARGET EXPERIMENTAL MODELS

Rashmi Das and Sunita Bhatnagar*

ABSTRACT

Nyctanthus arbortristis leaf extracts were subjected to antifungal activity against Aspergillus flavus and Aspergillus niger. Four leaf extracts were prepared these were hexane, chloroform, ethyl acetate and methanol extracts. Three methods were used to assess the antifungal activity these were radical growth method, agar diffusion method and biomass reduction. Reduction in aflatoxin content was also observed using UV-vis spectropotometer. Amongst all the extracts ethyl extract exhibited good activity in all the methods. Ethyl acetate extract was able to reduce the aflatoxin content by 42% when compared with control samples. At higher dose of 1mg/ml same extract inhibited the growth of fungus (A.flavus) by 60% and 40% in Aspergillus niger on day 2 in radical growth method.

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