World Journal of
Pharmaceutical and Life Sciences

PRODUCTION AND OPTIMIZATION OF PECTINASE AND CHITINASE PRODUCING BACTERIAL STRAINS ISOLATED FROM MANGROVE SEDIMENTS OF NIJAMPATNAM

G. Venkatalakshmi*

ABSTRACT

A total of ten bacterial isolates were used for the production of chitinase and protease and the soil samples were collected from mangrove areas of Nijampatnam, Andhra Pradesh. Isolation procedures were carried out by using nutrient agar medium (NAM). Marine water was used for the enzyme production and bacterial isolation. Chitin broth media and pectin broth media were used for this study. The present ten isolates are Pseudomonas, Staphylococcus, Micrococci, Klebsiella sp.1 Klebsiella sp.2, E. coli, Enterobacter, Proteus sp.1, Proteus sp.2, and Citrobacter are morphologically different. All these strains were tested for chitinase and Pectinase production. Maximum chitinase production (1.21 IU/ml) was observed by Enterobacter sps. The other isolate pseudomonas produces maximum amount (1.19 IU/ml) of pectinase. These two isolates were selected for the further studies like bio control agents. The optimum conditions pH 7.0, temperature 30 C and incubation period 48 hours for maximum production of enzyme. The carbon and nitrogen sources glucose and peptone greatly influenced the enzyme production. These enzymes have received increased attention due to its wide range of biotechnological applications, especially in agriculture for bio control of phytopathogenic fungi and harmful insects.

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