World Journal of
Pharmaceutical and Life Sciences

GENETIC IDENTIFICATION AND ANTAGONISTIC EFFECTS OF BIFIDOBACTERIUM ISOLATED FROM AL-JABAL AL-AKHDAR HONEY SAMPLES AGAINST HUMAN PATHOGENS

*Dr. Nagea Abdalsadiq

ABSTRACT

Probiotics are described as live bacteria that, when given in sufficient concentrations, provide health advantages to the host. Probiotic bacteria are the favored microorganism to a diversity of industries. The main purpose of this work was to screen probiotic bacteria isolated from honey for their antagonistic effects against resistance pathogen and characterization them using 16s rDNA. The results showed that, the probiotic bacteria were able to prevent growth of pathogens and exhibited significant inhibition of the growth of the Gram positive and Gram-negative bacteria growth as assessed using the microtiter plate. The four bacteria strains were identified three Lactiplantibacillus plantarum and one Bifidobacterium hapali tyrs. This study concluded that these secondary metabolite from probiotic bacteria can be used as a natural antimicrobial agent, added to food formulations to prevent of pathogenic microorganism’s growth and can be used as a preventive strategy to delay the onset of pathogenic biofilm growth on catheters and other medical insertional materials, reducing the use of synthetic drugs and chemicals.

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