Ashraf Unnisa*, Dr. Osman Ahmed, Meher Afrin, Mohammed Akthar Sulthana and Dr. Anas Rasheed

World Journal of
Pharmaceutical and Life Sciences

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Abstract

EVALUATION AND VALIDATION OF A UPLC METHOD FOR ESTIMATION OF ALBENDAOLE IN VETERINARY DOSAGE FORM

Ashraf Unnisa*, Dr. Osman Ahmed, Meher Afrin, Mohammed Akthar Sulthana and Dr. Anas Rasheed

ABSTRACT

In order to achieve the separation under the optimized conditions after experimental trials that can be summarized. Stationary phase like Hypersil BDS C18 (100 mm x 2.1 mm, 1.7 ?m) column was most suitable one, since it produced symmetrical peaks with high resolution and a very good sensitivity and with good resolution. The flow rate was maintained 1.5 mL min-1 shows good resolution. The PDA detector response of Albendaole was studied and the best wavelength was found to be 225 nm showing highest sensitivity. The mixture of two solutions Water - acetonitrile in the ratio of 70:30%v/v”. Finally, the pH was adjusted to 7.65 by sodium hydroxide. with gradient programming was used as mobile phase at 1.5mL/min was found to be an appropriate mobile phase for separation of Albendaole. The column was maintained at ambient temperature.

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