World Journal of
Pharmaceutical and Life Sciences

SIMULTANEOUS ESTIMATION OF TRIGONELLINE HYDROCHLORIDE AND BERBERINE DIHYDRATE BY USING HPTLC METHOD FROM HF/R/AD/310 POLYHERBAL ANTIDIABETIC TABLET

Unmesh Keshwar*, Deepmala Matey, Kashish Dhanrajani, Prajakta Pakade, Sharad Dhurde, B. K. Shrikhande

ABSTRACT

Most of the herbal formulations available in the market widely used for the management of diabetes are not standardized with respect to its active ingredients marker molecule. The present study was designed with an objective of simultaneous estimation of Trigonelline hydrochloride and Berberine dihydrate by HPTLC from HF/R/AD/310 Polyherbal Antidiabetic Tablet. Chromatographic separation was achieved on pre-coated silica gel TLC aluminium plate 60 F254 using Ethyl Acetate: Methanol: Water: Glacial Acetic acid, 3:6:2:1 (v/v/v/v) as mobile phase followed by densito-metric measurement at two different wavelength 268 nm and 348 nm by TLC Scanner 4. The retention factors of Trigonelline hydrochloride and Berberine dihydrate was 0.25 and 0.66 respectively. The estimated amount of Trigonelline hydrochloride and Berberine dihydrate was found to be 0.02 % and 0.06 %. Results obtained in validation parameters indicate the accuracy and reliability of the developed simultaneous HPTLC method. The proposed method found to be simple, sensitive, selective and accurate for routinely used in Quality Control Lab.

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