World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
World Journal of Pharmaceutical and Life Sciences (WJPLS) has indexed with various reputed international bodies like : Google Scholar, Index Copernicus, Indian Science Publications, SOCOLAR, China, Cosmos Impact Factor, Research Bible, Fuchu, Tokyo. JAPAN, Scientific Indexing Services (SIS), Jour Informatics (Under Process), UDLedge Science Citation Index, Global Impact Factor (In Process), International Impact Factor Services, International Scientific Indexing, UAE, International Society for Research Activity (ISRA) Journal Impact Factor (JIF), Science Library Index, Dubai, United Arab Emirates, International Innovative Journal Impact Factor (IIJIF), Scientific Journal Impact Factor (SJIF), Eurasian Scientific Journal Index (ESJI), Indian citation Index (ICI), IFSIJ Measure of Journal Quality, International Scientific Indexing, UAE (ISI), Web of Science Group (Under Process), Directory of Research Journals Indexing, Scholar Article Journal Index (SAJI), International Scientific Indexing ( ISI ), 

Abstract

ENHANCEMENT OF COATING STABILITY FOR CAPILLARY ZONE ELECTROPHORESIS OF PROTEINS IN DDAB MODIFIED SURFACES

Anisa Elhamili*, Douglas Westerlund, Jonas Bergquist and Stellan Hjertén

ABSTRACT

Separation of basic proteins with capillary electrophoresis was performed using a cationic double chain N, N-didodecyl-N, N-dimethylammonium bromide (DDAB) as coating reagent for fused silica capillaries and ammonium acetate as background electrolyte (BGE) at pH 4.0, 70 mM. This double-chained surfactant forms a semi-stable coating that provides a strong electro-osmosis flow (EOF) toward the anode. To maintain the highest efficiency and reproducibility, such coating must regularly be regenerated. The temporal stability of the coating was improved by a new procedure which increases the hydrophobic interaction between the DDAB coating and the silica wall and thus makes the coating more stable and at the same time the electrostatically adsorbed proteins are released. The procedure includes rinsing the capillaries, which were kept overnight in 1.0 mM DDAB solution, with (1:1 v/v) 0.5 M NaCl in the BGE. Rinsing the capillaries with this solution gave a stable coating which permitted the separation of proteins for up to 3 consecutive days without recoating and with maintained good precision in migration times of the analytes and EOF measurements compared to the previously published method in which the capillary can be used for 9 hours only.

[Full Text Article]

BEST PAPER AWARDS

World Journal of Pharmaceutical and life sciences Will give best paper award in every issue in the from of money along with certificate to promote research .

Best Article of current issue

Download Article : Click here