World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

DETERMINATION OF RETENTION FACTOR IN PIGMENTS OF PETAL

Manwar N. and Maruti S. Darade*

ABSTRACT

The investigation was carried out on separation of pigments from petals of flower of different plants. The five flowering plants such as Ixora, Portulaca, Hibiscus, Clitoria and Lilium were selected for pigment separation.The paper chromatography technique was used for separation of pigments.The separation of coloured pigments was observed and its values were recorded on the basis of distance traveled by specific pigment along with solvent. The distance of pigments traveled was measured in centimeters .The variation in retention factor was recorded in all the five plants The distance traveled by stationery phase and mobile phase on chromatography paper was used to determine retention factor ( Rf) of pigments, in all the flower of selected plants .The Rf of was noted in Ixora (0.58) , Portulaca (0.53) , Hibiscus (0.72),Clitoria (0.69) and Lilium (0.79) The minimum Rf ( 0.53) was noted in petals of Portulaca flower and maximum Rf (0.79) in petals of Lilium flower.

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