World Journal of
Pharmaceutical and Life Sciences

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

EFFECT OF PHYSICAL FACTORS ON LIPASE ENZYME PRODUCTION BY SEED BORNE FUNGI OF GROUNDNUT

Deshmukh R. S.*

ABSTRACT

During present investigation total eleven seed borne fungi were isolated from five different varieties of groundnut by moist blotter plate and agar plate method of which six dominant seed borne fungi were screened to study effect of physical factors such as incubation period, pH and temperature on lipase production. From the results it is evident that lipase activity of seed borne fungi was optimum at 9th day of incubation period in most of the test fungi. Rhizopus nigricans showed maximum lipase production while Sclerotium rolfsii produced least lipase. Maximum lipase production was observed in Rhizopus nigricans and Fusarium oxysporum in Groundnut variety TAG-24 while in SB-XI maximum lipase production was observed in Rhizopus nigricans and Macrophomina phaseolina at pH 6.5. It was also observed that as pH increased, the lipase production decreased. Similarly, it was noticed that all fungal isolates produced more lipase at 30°C temperature.

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